Collaboration between CYANOTOPE AS Norway and Dr. Virginie MOLLE team

With the strong microbiology expertise of Dr. Virginie Molle and her team (LPHI), especially regarding bacterial pathogenicity, along with the access to the GénomiX and the GenSeq platform, we are hoping to fulfill the 3 objectives of this first collaborative project.

1 – Proof of concept for qPCR on 16S        

Deliverable: Comparison report
The first objective of this project is to provide a proof of concept for the currently provided tests. As mentioned earlier, two companies in Norway provide a test against Pasteurella sp. in Lumpsucker. Both use qPCR methods against the 16S of the bacteria. Our first objective is to reproduce this test and compare the results we obtain to those of one of the competitors. To do so, our partner and future customer Norsk oppdrettsservice AS has been collecting samples and divided each of them into two, sending half of it to their actual supplier and keeping the other half for the purpose of his project.
The 4 steps of this first objective are:

  • To develop a robust method to isolate and cultivate Pasteurella sp. from fish samples (kidney)
  • To consolidate the 16S extraction protocol, probably with kits
  • To establish the best technic and method for detecting the 16S by qPCR
  • To compare results obtained with the competition ones.

3 – Development of a new diagnostic test        

Deliverable: New diagnostic protocol
Our last objective, on a more R&D approach, we wish to be able to develop a new diagnostic test. By detecting/quantifying a different gene, marker of pathogenicity if possible, this new test will be developed with the aim of increasing its specificity and its robustness while keeping the costs, the duration and the complexity quite low.

LPHI  Laboratory of Pathogen Host Interactions
UMR 5235 - Université Montpellier
Place Eugène Bataillon, Bât. 24, CC107, 2ème étage
34095 Montpellier cedex 5

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