Theme 3 - Laila Gannoun

THEME 3: role of post-translational modifications in intracellular bacterial survival

Theme leader : Dr. Laila GANNOUN

Our projects investigate the involvement of post-translational modifications (PTM) in bacterial pathogenesis and host–pathogen interactions. As PTMs play fundamental roles in cellular physiology, it is not surprising that pathogens interfere in many different ways with the PTMs of their host to promote their own survival and replication. We aim to decipher the mechanisms developed by the pathogenic bacteria, S. aureus, to survive inside host cells. We focus on host adaptation during macrophage infection with S. aureus based on post-translational modifications.

 

First, we study secreted bacterial signalling proteins such as the low molecular weight protein tyrosine phosphatases (LMW-Ptps) PtpA and PtpB, in order to understand their role(s) during infection and/or adaptation of S. aureus to host environmental stress, leading to yet unexplored S. aureus modes of host-pathogen interactions such as the modulation of phosphorylation status of host signalling networks

 

Moreover, we explore  the  role  of SUMOylation, a novel PTM involved in bacterial intracellular survival. This PTM can modify host proteins and/or bacterial secreted proteins after host cell infection. Our goal is to (i) decipher SUMOylation regulation of secreted virulence factors and/or host proteins involved in S. aureus intracellular survival and virulence, (ii) identify and characterize SUMO-modified proteome (or SUMOylome) during infection, and (ii) target SUMOylation of host proteins or secreted during infection to develop anti-virulence strategies.


LPHI  Laboratory of Pathogen Host Interactions
UMR 5235 - Université Montpellier
Place Eugène Bataillon, Bât. 24, CC107, 2ème étage
34095 Montpellier cedex 5

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