Publications – Group M. Lebrun
Selected publications
Najm R, Ruivo MTG, Penarete-Vargas DM, Hamie M, Mouveaux T, Gissot M, Boulanger MJ, El Hajj H, Lebrun M (2023) Invasion of Toxoplasma gondii bradyzoites: Molecular dissection of the moving junction proteins and effective vaccination targets. Proc Natl Acad Sci USA 120:e2219533120. doi:10.1073/pnas.2219533120
Najm R, Hamie M, Berry-Sterkers L, Lebrun M, El Hajj H, Lamarque MH (2022)Toxoplasma Shelph, a Phosphatase Located in the Parasite Endoplasmic Reticulum, Is Required for Parasite Virulence. mSphere 7:e0035022. doi:10.1128/msphere.00350-22
Sparvoli D, Delabre J, Penarete-Vargas DM, Kumar Mageswaran S, Tsypin LM, Heckendorn J, Theveny L, Maynadier M, Mendonça Cova M, Berry-Sterkers L, Guérin A, Dubremetz JF, Urbach S, Striepen B, Turkewitz AP, Chang YW, Lebrun M (2022) An apical membrane complex for triggering rhoptry exocytosis and invasion in Toxoplasma. EMBO J 41:e111158. doi:10.15252/embj.2022111158
Cova MM, Lamarque MH, Lebrun M (2022) How Apicomplexa Parasites Secrete and Build Their Invasion Machinery. Annu Rev Microbiol 76:619-640. doi:10.1146/annurev-micro-041320-021425
Martinez M, Chen WD, Cova MM, Molnár P, Mageswaran SK, Guérin A, John ARO, Lebrun M, Chang YW (2022) Rhoptry secretion system structure and priming in Plasmodium falciparum revealed using in situ cryo-electron tomography. Nat Microbiol 7:1230-1238. doi:10.1038/s41564-022-01171-3
Mageswaran SK, Guérin A, Theveny LM, Chen WD, Martinez M, Lebrun M, Striepen B, Chang YW (2021) In situ ultrastructures of two evolutionarily distant apicomplexan rhoptry secretion systems. Nat Commun 12:4983. doi:10.1038/s41467-021-25309-9
Sparvoli D, Lebrun M (2021) Unraveling the Elusive Rhoptry Exocytic Mechanism of Apicomplexa. Trends Parasitol 37:622-637. doi:10.1016/j.pt.2021.04.011
Aquilini E, Cova MM, Mageswaran SK, Dos Santos Pacheco N, Sparvoli D, Penarete-Vargas DM, Najm R, Graindorge A, Suarez C, Maynadier M, Berry-Sterkers L, Urbach S, Fahy PR, Guérin AN, Striepen B, Dubremetz JF, Chang YW, Turkewitz AP, Lebrun M (2021) An Alveolata secretory machinery adapted to parasite host cell invasion.
Nat Microbiol 6:425-434. doi:10.1038/s41564-020-00854-z
Paul AS, Miliu A, Paulo JA, Goldberg JM, Bonilla AM, Berry L, Seveno M, Braun-Breton C, Kosber AL, Elsworth B, Arriola JSN, Lebrun M, Gygi SP, Lamarque MH, Duraisingh MT (2020) Co-option of Plasmodium falciparum PP1 for egress from host erythrocytes. Nat Commun 11:3532. doi:10.1038/s41467-020-17306-1
Wilson SK, Heckendorn J, Martorelli Di Genova B, Koch LL, Rooney PJ, Morrissette N, Lebrun M, Knoll LJ (2020) A Toxoplasma gondii patatin-like phospholipase contributes to host cell invasion. PLoS Pathog 16:e1008650. doi:10.1371/journal.ppat.1008650
Suarez C, Lodoen MB, Lebrun M (2020) Assessing Rhoptry Secretion in T. gondii. Methods Mol Biol 2071:143-155. doi:10.1007/978-1-4939-9857-9_8
Semenovskaya K, Lévêque MF, Berry L, Bordat Y, Dubremetz JF, Lebrun M, Besteiro S (2020) TgZFP2 is a novel zinc finger protein involved in coordinating mitosis and budding in Toxoplasma. Cell Microbiol 22:e13120. doi:10.1111/cmi.13120
Suarez C, Lentini G, Ramaswamy R, Maynadier M, Aquilini E, Berry-Sterkers L, Cipriano M, Chen AL, Bradley P, Striepen B, Boulanger MJ, Lebrun M (2019) A lipid-binding protein mediates rhoptry discharge and invasion in Plasmodium falciparum and Toxoplasma gondii parasites. Nat Commun 10:4041. doi:10.1038/s41467-019-11979-z
Berry L, Chen CT, Francia ME, Guerin A, Graindorge A, Saliou JM, Grandmougin M, Wein S, Bechara C, Morlon-Guyot J, Bordat Y, Gubbels MJ, Lebrun M, Dubremetz JF, Daher W (2018) Toxoplasma gondii chromosomal passenger complex is essential for the organization of a functional mitotic spindle: a prerequisite for productive endodyogeny. Cell Mol Life Sci 75:4417-4443. doi:10.1007/s00018-018-2889-6
Morlon-Guyot J, El Hajj H, Martin K, Fois A, Carrillo A, Berry L, Burchmore R, Meissner M, Lebrun M, Daher W (2018) A proteomic analysis unravels novel CORVET and HOPS proteins involved in Toxoplasma gondii secretory organelles biogenesis. Cell Microbiol 20:e12870. doi:10.1111/cmi.12870
Wengelnik K, Daher W, Lebrun M (2018) Phosphoinositides and their functions in apicomplexan parasites. Int J Parasitol 48:493-504. doi:10.1016/j.ijpara.2018.01.009
Lebrun M, Blanchard N (2017) Editorial overview: Host-microbe interactions: parasites. Curr Opin Microbiol 40:viii-xi. doi:10.1016/j.mib.2017.11.028
Guérin A, El Hajj H, Penarete-Vargas D, Besteiro S, Lebrun M (2017) RON4(L1) is a new member of the moving junction complex in Toxoplasma gondii. Sci Rep 7:17907. doi:10.1038/s41598-017-18010-9
Lentini G, El Hajj H, Papoin J, Fall G, Pfaff AW, Tawil N, Braun-Breton C, Lebrun M (2017) Characterization of Toxoplasma DegP, a rhoptry serine protease crucial for lethal infection in mice. PLoS One 12(12):e0189556. doi:10.1371/journal.pone.0189556
Frénal K, Dubremetz JF, Lebrun M, Soldati-Favre D (2017) Gliding motility powers invasion and egress in Apicomplexa. Nat Rev Microbiol 15(11):645-660. doi:10.1038/nrmicro.2017.86
Guérin A, Corrales RM, Parker ML, Lamarque MH, Jacot D, El Hajj H, Soldati-Favre D, Boulanger MJ, Lebrun M (2017) Efficient invasion by Toxoplasma depends on the subversion of host protein networks. Nat Microbiol 2:1358-1366. doi:10.1038/s41564-017-0018-1
Miliu A, Lebrun M, Braun-Breton C, Lamarque MH (2017) Shelph2, a bacterial-like phosphatase of the malaria parasite Plasmodium falciparum, is dispensable during asexual blood stage. PLoS One 12:e0187073. doi:10.1371/journal.pone.0187073
Nguyen HM, El Hajj H, El Hajj R, Tawil N, Berry L, Lebrun M, Bordat Y, Besteiro S (2017) Toxoplasma gondii autophagy-related protein ATG9 is crucial for the survival of parasites in their host. Cell Microbiol 19(6). doi:10.1111/cmi.12712
Berry L, Chen CT, Reininger L, Carvalho TG, El Hajj H, Morlon-Guyot J, Bordat Y, Lebrun M, Gubbels MJ, Doerig C, Daher W (2016) The conserved apicomplexan Aurora kinase TgArk3 is involved in endodyogeny, duplication rate and parasite virulence. Cell Microbiol 18:1106-20. doi:10.1111/cmi.12571
Daher W, Morlon-Guyot J, Alayi TD, Tomavo S, Wengelnik K, Lebrun M (2016) Identification of Toxoplasma TgPH1, a pleckstrin homology domain-containing protein that binds to the phosphoinositide PI(3,5)P2. Mol Biochem Parasitol 207(1):39-44. doi:10.1016/j.molbiopara.2016.03.011
Parker ML, Penarete-Vargas DM, Hamilton PT, Guérin A, Dubey JP, Perlman SJ, Spano F, Lebrun M*, Boulanger MJ* (2016) Dissecting the interface between apicomplexan parasite and host cell: Insights from a divergent AMA-RON2 pair. Proc Natl Acad Sci USA 113:398-403. doi:10.1073/pnas.1515898113. *equal contribution
Wang K, Peng ED, Huang AS, Xia D, Vermont SJ, Lentini G, Lebrun M, Wastling JM, Bradley PJ (2016) Identification of Novel O-Linked Glycosylated Toxoplasma Proteins by Vicia villosa Lectin Chromatography. PLoS One 11:e0150561. doi:10.1371/journal.pone.0150561
Delgadillo RF, Parker ML, Lebrun M, Boulanger MJ, Douguet D (2016) Stability of the Plasmodium falciparum AMA1-RON2 Complex Is Governed by the Domain II (DII) Loop. PLoS One 11:e0144764. doi:10.1371/journal.pone.0144764
Pihan E, Delgadillo RF, Tonkin ML, Pugnière M, Lebrun M, Boulanger MJ, Douguet D (2015) Computational and biophysical approaches to protein-protein interaction inhibition of Plasmodium falciparum AMA1/RON2 complex. J Comput Aided Mol Des 29:525-39. doi:10.1007/s10822-015-9842-7
Rugarabamu G, Marq JB, Guérin A, Lebrun M, Soldati-Favre D (2015) Distinct contribution of Toxoplasma gondii rhomboid proteases 4 and 5 to micronemal protein protease 1 activity during invasion. Mol Microbiol 97:244-62. doi:10.1111/mmi.13021
Lentini G, Kong-Hap M, El Hajj H, Francia M, Claudet C, Striepen B, Dubremetz JF, Lebrun M (2015) Identification and characterization of Toxoplasma SIP, a conserved apicomplexan cytoskeleton protein involved in maintaining the shape, motility and virulence of the parasite. Cell Microbiol 17:62-78. doi:10.1111/cmi.12337
Morlon-Guyot J, Pastore S, Berry L, Lebrun M, Daher W (2015) Toxoplasma gondii Vps11, a subunit of HOPS and CORVET tethering complexes, is essential for the biogenesis of secretory organelles. Cell Microbiol 17:1157-78. doi:10.1111/cmi.12426
Daher W, Morlon-Guyot J, Sheiner L, Lentini G, Berry L, Tawk L, Dubremetz JF, Wengelnik K, Striepen B, Lebrun M (2015) Lipid kinases are essential for apicoplast homeostasis in Toxoplasma gondii. Cell Microbiol 17:559-78. doi:10.1111/cmi.12383
Risco-Castillo V, Topçu S, Marinach C, Manzoni G, Bigorgne AE, Briquet S, Baudin X, Lebrun M, Dubremetz JF, Silvie O (2015) Malaria Sporozoites Traverse Host Cells within Transient Vacuoles. Cell Host Microbe 18(5):593-603. doi:10.1016/j.chom.2015.10.006
Lamarque MH, Roques M, Kong-Hap M, Tonkin ML, Rugarabamu G, Marq JB, Penarete-Vargas DM, Boulanger MJ, Soldati-Favre D, Lebrun M (2014) Plasticity and redundancy among AMA-RON pairs ensure host cell entry of Toxoplasma parasites. Nat Commun 5:4098. doi:10.1038/ncomms5098
Morlon-Guyot J, Berry L, Chen CT, Gubbels MJ, Lebrun M, Daher W (2014) The Toxoplasma gondii calcium-dependent protein kinase 7 is involved in early steps of parasite division and is crucial for parasite survival. Cell Microbiol 16:95-114. doi:10.1111/cmi.12186
Kong-Hap MA, Mouammine A, Daher W, Berry L, Lebrun M, Dubremetz JF, Besteiro S (2013) Regulation of ATG8 membrane association by ATG4 in the parasitic protist Toxoplasma gondii. Autophagy 9:1334-48. doi:10.4161/auto.25189
Tonkin ML, Crawford J, Lebrun ML, Boulanger MJ (2013) Babesia divergens and Neospora caninum apical membrane antigen 1 structures reveal selectivity and plasticity in apicomplexan parasite host cell invasion. Protein Sci 22:114-27. doi:10.1002/pro.2193
De Napoli MG, de Miguel N, Lebrun M, Moreno SN, Angel SO, Corvi MM (2013) N-terminal palmitoylation is required for Toxoplasma gondii HSP20 inner membrane complex localization. Biochim Biophys Acta 1833:1329-37. doi:10.1016/j.bbamcr.2013.02.022
Lebrun M, Carruthers VB, Cesbron-Delauw MF (2013) Toxoplasma Secretory Proteins and their roles in Cell Invasion and Intracellular Survival. in Toxoplasma gondii: The Model Apicomplexan – Perspectives and Methods, Weiss and Kim (Eds), 2nd edition, pp.265-315.
Dubremetz JF, Lebrun M (2012) Virulence factors of Toxoplasma gondii. Microbes Infect 14:1403-10. doi:10.1016/j.micinf.2012.09.005
Lamarque MH, Papoin J, Finizio AL, Lentini G, Pfaff AW, Candolfi E, Dubremetz JF, Lebrun M (2012) Identification of a new rhoptry neck complex RON9/RON10 in the Apicomplexa parasite Toxoplasma gondii. PLoS One 7:e32457. doi:10.1371/journal.pone.0032457
Vulliez-Le Normand B, Tonkin ML, Lamarque MH, Langer S, Hoos S, Roques M, Saul FA, Faber BW, Bentley GA, Boulanger MJ, Lebrun M (2012) Structural and functional insights into the malaria parasite moving junction complex. PLoS Pathog 8:e1002755. doi:10.1371/journal.ppat.1002755
Tawk L, Dubremetz JF, Montcourrier P, Chicanne G, Merezegue F, Richard V, Payrastre B, Meissner M, Vial HJ, Roy C, Wengelnik K, Lebrun M (2011) Phosphatidylinositol 3-monophosphate is involved in toxoplasma apicoplast biogenesis. PLoS Pathog 7:e1001286. doi:10.1371/journal.ppat.1001286
Besteiro S, Dubremetz JF, Lebrun M (2011) The moving junction of apicomplexan parasites: a key structure for invasion. Cell Microbiol 13:797-805. doi:10.1111/j.1462-5822.2011.01597.x
Lamarque M, Besteiro S, Papoin J, Roques M, Vulliez-Le Normand B, Morlon-Guyot J, Dubremetz JF, Fauquenoy S, Tomavo S, Faber BW, Kocken CH, Thomas AW, Boulanger MJ, Bentley GA, Lebrun M (2011) The RON2-AMA1 interaction is a critical step in moving junction-dependent invasion by apicomplexan parasites. PLoS Pathog 7(2):e1001276. doi:10.1371/journal.ppat.1001276
Hajagos BE, Turetzky JM, Peng ED, Cheng SJ, Ryan CM, Souda P, Whitelegge JP, Lebrun M, Dubremetz JF, Bradley PJ (2012) Molecular dissection of novel trafficking and processing of the Toxoplasma gondii rhoptry metalloprotease toxolysin-1. Traffic 13(2):292-304. doi:10.1111/j.1600-0854.2011.01308.x
Tonkin ML, Roques M, Lamarque MH, Pugnière M, Douguet D, Crawford J, Lebrun M*, Boulanger MJ* (2011) Host cell invasion by apicomplexan parasites: insights from the co-structure of AMA1 with a RON2 peptide. Science 333(6041):463-7. doi:10.1126/science.1204988. *equal contribution